How to remove a file lock manually in MS Server 2008
Open Administrative Tools -> Share and Storage Management.
From the Actions pane, click Manage Open Files.
All locked files and folders should be visible on this server.
To clear a lock, select the relevant file and click “Close Selected”.
Note only use this method if other option to close / unlock a file have been tried and a user definitely not using the the file, otherwise data loss may occur.